Getting Started with RNA-Seq Webinar Series
Whole transcriptome analysis is of growing importance in understanding how the altered expression of genetic variants contributes to complex diseases such as cancer, diabetes, and heart disease. RNA sequencing (RNA-Seq) provides researchers with greater insights into biological pathways and molecular mechanisms that may regulate development and disease. Learn more about RNA-Seq basics in our webinar series from those with transcriptome sequencing experience. Our 20-minute presentations will give you a better understanding of the application, experimental design, and data analysis options.
Webinar 1: Getting Started with Transcriptome Sequencing: An Introduction to RNA-Seq
Learn what RNA-Seq is and why it is a popular replacement for microarray applications
Webinar 2: Getting Started with Transcriptome Sequencing Experimental design overview
Learn how to select from the different approaches for RNA sequencing, and tips and tricks for designing your experiment. Learn which kits are right for your research.
Webinar 3: Whole Transcriptome Analysis Made Simple with Ion Torrent and Partek Flow Software
Our colleagues at Partek Flow will go over data analysis options and reports available without the need for a bioinformatician in your lab
Webinar 4: Getting Started with Transcriptome Sequencing: How to perform RNA-Seq Data Analysis
Learn how RNA-Seq data is generated and what other options you have for analysis besides Partek Flow Software. Find out tips and tricks to help you to get to your answer faster.
Customer success with RNA-Seq on the Ion Proton™ System Series
Webinar 1: RNA Sequencing for Biomarker Discovery
Audrey PappTechnical Director, The Pharmacogenomics Core Laboratory
The Ohio State University
Audrey Papp is a research specialist in pharmacology with expertise in pharmacogenomics and molecular pathology. As Technical Director of the Pharmacogenomics Core Laboratory at Ohio State University, she is instrumental in developing new methods and approaches geared towards discovery of functional genetic biomarkers. She has played a leading role in implementation of next-generation sequencing in the Pharmacogenomics Core Laboratory. The Pharmacogenomics Core Lab is currently using both the Ion PGM™ and Ion Proton™ Systems for next-generation sequencing
In this webinar Audrey Papp will discuss:
- Allelic expression as a tool for DNA variant detection
- Complex RNA interactions
- Whole transcriptome sequencing on the Ion Proton™ System for biomarker discovery
- Targeted RNA sequencing on the Ion PGM™ System using Ion AmpliSeq™ RNA technology
Webinar 2: Total Transcriptome Analysis on the Ion Torrent™ Platform
Timothy Triche, MD, PhDProfessor, Pathology & Pediatrics, USC KSOM
Director, Center for Personalized Medicine at CHLA
Dr. Triche received his AB in physics and biology from Cornell University, an MD and PhD degree from Tulane University Medical Center, and his pathology training at Washington University Medical Center. He was the PI on both NCI Director's Challenge and SPECS grants to develop diagnostic and prognostic/predictive RNA expression profiles for childhood cancer, and co-founded GenomeDx, a company using similar technology to predict clinical recurrence of prostate cancer after prostatectomy. He is the Chair Emeritus of the Pathology Department at Children's Hospital Los Angeles and serves as Director of the Center for Personalized Medicine at CHLA, as well as co-Director of the Institute for Molecular Medicine, a collaborative effort between Phoenix Children's Hospital and TGen.In this webinar Dr. Trische will discuss:
- An introduction to RNA-Seq
- Total RNA-Seq vs. poly A RNA-Seq
- RNA transcripts in tumor research
Webinar 3: Performing transcriptome Sequencing on the Ion Proton System
Associate Director of Applications
Thermo Fisher Scientific
Sequencing is a powerful tool for looking at the complexity of the transcriptome. This webinar will discuss considerations for preparing your transcriptome sequencing experiments along with the use of Life Technologies solutions including the Ion Proton™ System. Both upfront sample preparation from Ambion and downstream data analysis solutions from Partek will be described.