Optimize your PCR

The polymerase chain reaction (PCR) is one of the most widespread methods used in molecular biology laboratories and clinical research. In recent years, PCR has become very convenient and easy to perform due to the availability of ready-to-use kits and user-friendly thermal cyclers. Despite these advances the basic rules of PCR have remained the same, and having a thorough understanding of them is essential to running PCR successfully. In this webinar we will review the fundamentals of PCR and discuss best practices for reaction setup and optimization as well as data analysis.

Topics Covered

  • PCR: Back to basics
  • PCR reaction set up: Do’s and Don’ts
  • Essential controls in PCR
  • Enzymes for routine, high-fidelity and fast PCR
  • PCR data analysis and troubleshooting

About the presenter

Alexander Trampe, PhD
Senior Technical Application Scientist
Thermo Fisher Scientific

Dr. Alexander Trampe studied biochemistry for his undergraduate degree, then received his PhD in molecular biology. He joined Thermo Fisher Scientific more than 7 years ago as a Technical Support Specialist for PCR and qPCR tools and applications. Since then he has gathered extensive experience on the setup of the PCR workflow, its most common pitfalls and PCR data analysis. Dr. Trampe is now a Senior Technical Application Scientist supporting molecular biology, cell analysis and cell culture products.

 

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