Tips and Tricks for the Cloning Workflow
One of the most common techniques in molecular biology is molecular or DNA cloning. The molecular cloning workflow incorporates many different steps, including nucleic acid purification, restriction endonuclease digestion, DNA end modification, ligation, and transformation. Each of these steps requires the use of different enzymes and protocols, all of which need to perform efficiently for accurate results.
In this webinar we present important considerations as well as tips and tricks to simplify the cloning workflow. We cover the most frequent problems and cloning pitfalls, as well as innovative solutions that can reduce the time and resource requirements, and errors, in a typical cloning workflow.
Topics covered:
- Nucleic acid purification considerations
- How to choose PCR and RT enzymes for cloning
- Tips and tricks for restriction digestion
- How to optimize ligation
About the presenter
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Jennifer received her undergraduate degree in biology, followed by a Ph.D. in microbiology from Auburn University and postdoctoral research at Northwestern University. She has worked in the life science research tools industry for over 10 years. Jennifer joined Thermo Fisher Scientific 3 years ago as a marketing manager supporting the molecular biology portfolio of products, and enjoys making complex technical information more accessible to new users. |
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