Specific isolation and analysis of exosome sub-populations
Exosomes are small (30-120 nm) vesicles containing proteins, RNA and lipids, secreted by all cell types in culture, and found in most body fluids. Huge progress is being made to understand their biology and potential clinical use, including their role as biomarkers.
Exosomes are attractive for biomarker research for several reasons; i) their cargo is protected against nucleases and proteinases present in body fluids, ii) only live cells produce exosomes, and iii) surface markers can reflect their origin (e.g. tumor-derived).
Isolation is a challenging and important step in the characterization and study of exosomes. While ultracentrifugation allows for their isolation, this method cannot discriminate efficiently between subpopulations or other microvesicles.
In this webinar, Life Technologies scientists Axl Neurauter, Ketil Winter Pedersen, and Sasha Vlassov present an alternative and more versatile method for exosome islation; magnetic separation using Dynabeads® with antibodies against classical exosome surface proteins (CD9, CD63, CD81 and EpCAM). The exosome sub-populations captured with these magnetic beads are suited for downstream analysis such as: flow cytometry, ELISA, mass spectrometry, electron microscopy, qRT-PCR, and sequencing.
We strongly believe tools for specific isolation and analysis of exosomes sub-populations will accelerate further research into these unique vesicles, and in the near future provide valuable tools in clinical settings.
Meet the scientists