Direct PCR: Amplify Without Purification

The direct PCR approach enables amplification from crude samples and eliminates the need for any DNA purification steps before PCR. This method significantly simplifies traditional PCR workflows, making it increasingly popular for basic and applied research applications such as genotyping, DNA sequence detection and sequencing. This webinar will review the basics of the direct PCR method, present most common applications and provide guidelines on how to perform direct PCR on different sample types.

Topics Covered

• Introduction to direct PCR approach and applications
• Inhibitor-tolerant DNA polymerases
• Tips and tricks for amplification without DNA purification
• Gentle genotyping with direct PCR

Meet the presenter

Marija Navickaite, Msc Product Manager, Molecular Biology Thermo Fisher Scientific Marija studied biochemistry for her undergrad degree, followed by Msc degree in Human Genetics. She joined Thermo Fisher Scientific as a R&D scientist and worked on development of new Thermo Scientific products. Marija is now Product Manager for Thermo Scientific Gene Detection portfolio and is responsible for several PCR-based product lines.